阿魏酸对巨噬细胞泡沫样化过程中胆固醇流出的影响及可能机制

主任药师，研究方向为中药药理学，Tel：13907119777， E-mail：whcoltene@sohu.comEffect of ferulic acid on cholesterol efflux in macrophage foam

cell formation and potential mechanismCHEN Fu-xin1*， WANG Lian-kai2

（1.Wuhan University， Wuhan 430072， China;  2. Hubei Institute for Nationalities Major Hospital Affiliated China， Enshi 445000， China）[Abstract]The formation of macrophage-derived foam cells is a typical feature of atherosclerosis （AS）. Reverse cholesterol efflux （RCT） is one of important factors for the formation of macrophage foam cells. In this study， macrophage form cells were induced by oxidized low density lipoprotein （ox-LDL） and then treated with different concentrations of ferulic acid， so as to observe the effect of ferulic acid on the intracellular lipid metabolism in the ox-LDL-induced macrophage foam cell formation， the cholesterol efflux and the mRNA expression and protein levels of ATP binding cassette transporter A1 （ABCA1） and ATP binding cassette transporter G1 （ABCG1） that mediate cholesterol efflux， and discuss the potential mechanism of ferulic acid in resisting AS. According to the findings， compared with the control group， the ox-LDL-treated group showed significant increase in intracellular lipid content， especially for the cholesterol content; whereas the intracellular lipid accumulation markedly decreased， after the treatment with ferulic acid. The data also demonstrated that the mRNA and protein expressions of ABCA1 and ABCG1 significantly increased after macrophage foam cells were treated with different concentrations of ferulic acid. In summary， ferulic acid may show the anti-atherosclerosis effect by increasing the surface ABCA1 and ABCG1expressions of macrophage form cells and promoting cholesterol efflux.

[Key words]ferulic acid; atherosclerosis; cholesterol efflux; ATP binding cassette transporter

doi：10.4268/cjcmm20150330

动脉粥样硬化（atherosclerosis， AS）是导致冠状动脉疾病发病和死亡的主要原因， 而巨噬细胞泡沫样改变是AS的特征性病理改变。泡沫样细胞的形成与细胞内脂质代谢异常密切相关，胆固醇摄入过多或者外流减少，均可引起脂质在细胞内的聚集而使巨噬细胞转化为泡沫样细胞^[1-3]。